Publication Types:

Event Dosimeter Devices and Methods Thereof

Patents
David A. Borkholder, Gregory TA Kovacs, Jeffrey Rogers
U.S. Patent No. 9,668,689 B2. 6 June 2017
Publication year: 2017

A method including a dosimetry device  having at least one sensor in a housing and a dosimetry processing device with a memory. The dosimetry processing device is coupled to the at least one sensor in the housing.  The dosimetry processing device is configured to execute programmed instructions stored in the memory comprising; obtaining readings from the sensor; storing the readings; conducting an analysis of the stored readings to determine an injury risk assessment;  and outputting at least one of the conducted analysis of the determined injury risk assessment or the stored readings.

Methods for Monitoring Exposure to an Event and Devices Thereof

Patents
D.A. Borkholder , D. DeBusschere
AU 2012222109, issued April 21, 2016
Publication year: 2016

Event dosimeter devices and methods thereof

Patents
David A. Borkholder, Gregory TA Kovacs, Jeffrey Rogers
U.S. Patent No. 9,339,224. 17 May 2016
Publication year: 2016

Devices, systems and methods for detecting and evaluating impact events

Patents
Borkholder DA, Kenyon M, Ramplin R, Ostertag MH, Sherman K, Wellman M, Harrison M
US Patent No 9,380,961, 5 July 2016
Publication year: 2016

Methods for Monitoring Exposure to an Event and Devices Thereof

Patents
Borkholder DA, DeBusschere D
Australian Patent 2012222109, Accepted 2015
Publication year: 2015

A method, non-transitory computer readable medium, and apparatus that includes obtaining, by a dosimetry computing device, sensor readings from at least one sensor. An event is identified, by the dosimetry computing device, based on at least one of one or more of the obtained sensor readings or one or more determinations based on the obtained sensor readings meeting one or more selection. At least one of the one or more de terminations or the sensor readings which meet one or more of the selection criteria when the event is identified is stored by the dosimetry computing device.

Au dosimeter 2

Method for Monitoring Exposure to an Event and Device Thereof

Patents
Borkholder DA, DeBusschere BD
U.S. Patent 9,138,172, issued September 22, 2015.
Publication year: 2015

A method, non-transitory computer readable medium, and apparatus that includes obtaining, by a dosimetry computing device, sensor readings from at least one sensor. An event is identified, by the dosimetry computing device, based on at least one of one or more of the obtained sensor readings or one or more determinations based on the obtained sensor readings meeting one or more selection. At least one of the one or more determinations or the sensor readings which meet one or more of the selection criteria when the event is identified is stored by the dosimetry computing device.

Method dosimeter

Event Monitoring Dosimetry Apparatuses and Methods Thereof

Patents
Borkholder DA, Fassler W, Blair A, Sherman K, DeBusschere D
Australian Patent 2012222107, Issued June 11, 2015
Publication year: 2015

A dosimetry apparatus includes at least one sensor in a housing, a cover configured to permit compression waves to pass through, the cover is seated over the at least one sensor, and a dosimetry processing device with a memory. The dosimetry pro CA cessing device is coupled to the at least one sensor in the housing. The dosimetry processing device is configured to execute programmed instructions stored in the memory comprising: obtaining readings from the at least one sensor; storing the readings with a time and date stamp when obtained; conducting an analysis based on the obtained readings; and outputting at least one of the stored readings or the conducted analysis.

Au dosimeter 3

Event Dosimeter Device and Methods Thereof

Patents
Borkholder DA, Kovacs GTA, Rogers J
Australian Patent 2012222110, Issued August 6, 2015
Publication year: 2015

A dosimetry device includes at least one sensor in a housing and a dosimetry processing device with a memory. The dosimetry processing device is coupled to the at least one sensor in the housing. The dosimetry processing device is configured to ex ecute programmed instructions stored in the memory comprising: obtaining readings from the sensor; storing the readings; conducting an analysis of the stored readings to determine an injury risk assessment; and outputting at least one of the conducted analysis of the de termined injury risk assessment or the stored readings.

Au Dosimeter

Device for Detecting and Impact Event

Patents
Borkholder DA, Kenyon M, Ramplin R, Sherman K, Wellman M, and Harrison M
U.S. Patent D743,822, Issued November 24, 2015
Publication year: 2015

The ornamental design of a device for detecting an impact event, as shown and described.

Linx design patent

Device for Detecting an Impact Event

Patents
DA Borkholder, M Kenyon, R Ramplin, K Sherman, M Wellman, and M Harrison
US D743,822, issued November 24, 2015
Publication year: 2015

Host Supported Genetic Biosensors

Patents
DA Borkholder
US 13/234,992, September 16, 2011
Publication year: 2011

Determining starting amount of target nucleotide sequences; amplify nucleotide sequences, amplify control, determine threshold points, calculate propagation curve, determine starting amount of nucleotide sequences.

Patents
McMillan, William A., Lee A. Christel, David A. Borkholder, and Steven J. Young
U.S. Patent 6,911,327, issued June 28, 2005
Publication year: 2005

A method for determining an unknown starting quantity of a target nucleic acid sequence in a test sample comprises amplifying the unknown starting quantity of the target nucleic acid sequence in the test sample and known starting quantities of a calibration nucleic acid sequence in respective calibration samples; and determining a respective threshold value for each of the nucleic acid sequences using a derivative of a growth curve derived for the sequence. The starting quantity of the target nucleic acid sequence in the test sample is determined using the threshold value determined for the target sequence and a calibration curve derived from the threshold values determined for the known starting quantities of the calibration nucleic acid sequences.

Determining-startingde-sequences

Apparatus for analysis of a nucleic acid amplification reaction.

Patents
Mcmillan, William A., Lee A. Christel, David A. Borkholder, and Steven J. Young
U.S. Patent 6,942,971, issued September 13, 2005.
Publication year: 2005

An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of a nucleic acid sequence being amplified in the reaction. A controller in communication with the detection mechanism is programmed to store signal values defining a growth curve for the nucleic acid sequence, determine a derivative of the growth curve, and calculate a cycle number or time value associated with a characteristic of the derivative.

Apparatus-for-analysis-of-a-nucleic-acid-amplification-reaction.

Useful for diagnosis, monitoring changing levels of viral nucleic acid in a patient throughout course of illness (hiv)

Patents
McMillan, William A., Lee A. Christel, David A. Borkholder, and Steven J. Young
U.S. Patent 6,783,934, issued August 31, 2004.
Publication year: 2004

A method for determining a threshold cycle number or time value in a nucleic acid amplification reaction comprising the steps of amplifying a nucleic acid sequence in a reaction mixture; at a plurality of different times during the amplification reaction, measuring at least one signal whose intensity is related to the quantity of the nucleic acid sequence in the reaction mixture; and storing signal values defining a growth curve for the nucleic acid sequence. A derivative of the growth curve is determined and the threshold cycle number or time value is calculated as a cycle number or time value corresponding to a characteristic (e.g., maximum, minimum, or zero-crossing) of the derivative.

Useful-for-diagnosis

Apparatus for quantitative analysis of a nucleic acid amplification reaction

Patents
McMillan, William A., Lee A. Christel, David A. Borkholder, and Steven J. Young.
U.S. Patent 6,713,297, issued March 30, 2004.
Publication year: 2004

An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of a nucleic acid sequence being amplified in the reaction. The apparatus also includes a controller in communication with the detection mechanism. The controller is programmed to perform the steps of deriving a growth curve from the measurements of the signal; calculating a derivative of the growth curve; identifying a characteristic of the derivative; and determining a threshold value associated with the characteristic of the derivative. Embodiments of an apparatus for determining a starting quantity of a nucleic acid sequence in a test sample are also provided.

 

Apparatus-for-quantitative-analysis-of-a-nucleic-acid-amplification-reaction

Classification of biological agents according to the spectral density signature of evoked changes in cellular electric potential

Patents
Borkholder, David
U.S. Patent 6,377,057, issued April 23, 2002.
Publication year: 2002

Biologically active compounds are classified according to their effect on ion channels, changes in membrane potential and ionic currents, and the frequency content of action potentials that the compound(s) evoke in excitable cells. The spectral density changes of such evoked membrane potential or action potential are a characteristic for each channel type that is modulated by the test compound. A pattern of spectral changes in membrane potential is determined by contacting a responsive cell with a compound, and monitoring the membrane potential or ionic currents over time. These changes correlate with the effect of that compound, or class of compounds, on the ion channels of the responding cell. This pattern of spectral changes provides a unique signature for the compound, and provides a useful method for characterization of channel modulating agents.

 

Classification-of-biological-agents

Hybrid Biosensors

Patents
Kovacs GTA, and Borkholder DA
US Patent 6,051,422, Issued April 18, 2000.
Publication year: 2000

The invention relates to an apparatus and method for monitoring cells and to a method for monitoring changes in cells upon addition of an analyte to the cell’s environment, comprising a device which includes an array of microelectrodes disposed in a cell culture chamber, upon which array a portion of cells adhere to the surfaces of the microelectrodes. The diameter of the cells are larger than the diameters of the microelectrodes. A voltage signal is applied across each of the microelectrodes and a reference electrode. Detection and monitoring of the signals resulting from the application of the voltage signal provides information regarding the electrical characteristics of the individual cells, including impedance (combined cell membrane capacitance and conductance), action potential parameters, cell membrane capacitance, cell membrane conductance, and cell/substrate seal resistance. Such an invention is useful in detecting or screening a variety of biological and chemical agents.

hybrid biosensors 2000

Hybrid biosensors

Patents
Kovacs, Gregory TA, and David A. Borkholder
U.S. Patent 5,981,268, issued November 9, 1999.
Publication year: 1999

The invention relates to an apparatus and method for monitoring cells and to a method for monitoring changes in cells upon addition of an analyte to the cell’s environment, comprising a device which includes an array of microelectrodes disposed in a cell culture chamber, upon which array a portion of cells adhere to the surfaces of the microelectrodes. The diameter of the cells are larger than the diameters of the microelectrodes. A voltage signal is applied across each of the microelectrodes and a reference electrode. Detection and monitoring of the signals resulting from the application of the voltage signal provides information regarding the electrical characteristics of the individual cells, including impedance (combined cell membrane capacitance and conductance), action potential parameters, cell membrane capacitance, cell membrane conductance, and cell/substrate seal resistance. Such an invention is useful in detecting or screening a variety of biological and chemical agents.

 

Hybrid-biosensors